Titlebook: RNA Therapeutics; Function, Design, an Mouldy Sioud Book 2010 Humana Press 2010 DNA.Microarray.PCR.Transplantat.Tumor.cloning.gene expressi

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Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells,ation, function, and survival can be modulated by small interfering RNAs (siRNAs) targeting genes involved in immune suppression. This chapter describes detailed protocols for introducing siRNAs into human monocytes and dendritic cells using standard electroporation techniques.

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Inhibition of the microRNA Pathway in Zebrafish by siRNA,this important vertebrate model because of unspecific developmental defects. We have recently shown that these side effects can be attributed to inhibition of the miRNA pathway by siRNAs at early embryonic stages. This review highlights these findings and the function of microRNAs in zebrafish development.

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RNA Modifications: A Mechanism that Modulates Gene Expression,and protein translation are two major steps in eukaryotic gene expression that necessitate the production of accurate gene products. Both processes occur in large complexes, consisting of both proteins and noncoding RNAs. Interestingly, the RNA components contain a large number of posttranscriptiona

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Quantitative Analysis of RNA Modifications, modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of te

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Progress in siRNA Delivery Using Multifunctional Nanoparticles,icles need to efficiently encapsulate siRNA, actively target sites of interest, and release siRNA intracellularly. This chapter reviews recent progress using a multifunctional approach to design and engineer polymeric nanoparticles for siRNA delivery.

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Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells, Unfortunately, its curative potential is in general limited by immunosuppressive proteins that negatively regulate dendritic (DC) and/or T-cell function. The recent discovery of RNA interference (RNAi) has facilitated the study of gene function in immune cells and recent data indicate that DC matur

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Using OligoWalk to Identify Efficient siRNA Sequences,ression of specific mRNAs. In practice, a number of factors influence whether an siRNA sequence will elicit RNAi and knockdown target gene expression. One factor that significantly influences the efficiency of an siRNA is the effect of RNA secondary structure. Self-structure in either the siRNA sequ