Titlebook: RNA Therapeutics; Function, Design, an Mouldy Sioud Book 2010 Humana Press 2010 DNA.Microarray.PCR.Transplantat.Tumor.cloning.gene expressi

繁荣地区

neolith

咒语

https://doi.org/10.1007/978-1-60761-657-3DNA; Microarray; PCR; Transplantat; Tumor; cloning; gene expression; genes; hybridization; miRNAs; molecular b

Heretical

Progress in siRNA Delivery Using Multifunctional Nanoparticles,icles need to efficiently encapsulate siRNA, actively target sites of interest, and release siRNA intracellularly. This chapter reviews recent progress using a multifunctional approach to design and engineer polymeric nanoparticles for siRNA delivery.

捐助

Functional Studies on RNA-Transfected Cell Microarrays,ays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates.

模仿

CRACK

Mortal

Blood-Vessels

Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1, three separate siRNA sequences targeting unique sites in HIV-1. This methodological overview explains some crucial aspects of lhRNA design and cloning as well as facile experiments to determine their efficacy in cell culture.

放弃

Profiling of miRNA Expression and Prediction of Target Genes,. This chapter highlights the technical advances in miRNA gene profiling and discusses the limitations of target prediction programs and the necessity of defining the roles that govern miRNA specificity and target discrimination in vivo.