书目名称 | RNA Modifications | 副标题 | Methods and Protocol | 编辑 | Mary McMahon | 视频video | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This detailed book describes some of the most recent advances and up-to-date methodologies to detect, quantify, analyze, and elucidate the biological function of different types of RNA modifications. Importantly, the methodologies and tools described herein can be applied to a wide variety of organisms and can be used to address biological and clinical questions. Beginning with a section on bioinformatics tools, the collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, qPCR- and molecular biology-based methods, mass spectrometry- and NMR-based methods, as well as approaches to assess kinetics, determinants, and functions of RNA modifications. Written for the highly successful .Methods in Molecular Biology. series style, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .RNA Modifications: Methods and Protocols. serves as an ideal guide for those working directly in the fields of epitranscriptomics a | 出版日期 | Book 2021 | 关键词 | Epitranscriptome; Post-transcriptional RNA modifications; Messenger RNA; Post-transcriptional gene regu | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-1374-0 | isbn_softcover | 978-1-0716-1376-4 | isbn_ebook | 978-1-0716-1374-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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