| 书目名称 | RNA Detection and Visualization | | 副标题 | Methods and Protocol | | 编辑 | Jeffrey E. Gerst | | 视频video | | | 概述 | Features methods and protocols relevant for both fixed and live cells from everything from bacteria to mammalian models.Provides detailed techniques from established and up-and-coming experts in the f | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In .RNA Detection and Visualization: Methods and Protocols., the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.. .Authoritative and cutting-edge, .RNA Detection and Visualization: Methods and Protocols. offers well- | | 出版日期 | Book 2011 | | 关键词 | Advanced live imaging; Affinity purification; Bioinformatic tools; Cell fractionation; In situ hybridiza | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-61779-005-8 | | isbn_softcover | 978-1-4939-5836-8 | | isbn_ebook | 978-1-61779-005-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2011 |
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发表于 2025-3-21 16:05:27
