| 书目名称 | Progenitor Cells | | 副标题 | Methods and Protocol | | 编辑 | Mugdha V. Joglekar,Anandwardhan A. Hardikar | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Progenitor Cells: Methods and Protocols. is a valuable resource for scientists and researchers interested in learni | | 出版日期 | Book 2019 | | 关键词 | Cell isolation; Cellular differentiation; Imaging techniques; Cellular characterization methods; Progeni | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-9631-5 | | isbn_softcover | 978-1-4939-9633-9 | | isbn_ebook | 978-1-4939-9631-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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发表于 2025-3-21 19:55:28
