书目名称 | Plant Functional Genomics |
副标题 | Methods and Protocol |
编辑 | Jose M. Alonso,Anna N. Stepanova |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This second edition volume. .discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in |
出版日期 | Book 2015Latest edition |
关键词 | DNA sequencing; RNA secondary structure; conformational changes; epigemetic changes; genome; genomics tec |
版次 | 2 |
doi | https://doi.org/10.1007/978-1-4939-2444-8 |
isbn_softcover | 978-1-4939-4913-7 |
isbn_ebook | 978-1-4939-2444-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2015 |