Titlebook: Non-Ribosomal Peptide Biosynthesis and Engineering; Methods and Protocol Michael Burkart,Fumihiro Ishikawa Book 2023 The Editor(s) (if appl

含铁

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恩惠

1064-3745 ation advice from the experts.This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistr

转折点

liaison

过剩

凹室

Chemical Labeling of Protein 4′-Phosphopantetheinylation in Surfactin-Producing Nonribosomal Peptidee chemical labeling of 4′-phosphopantethylated NRPSs. In this chapter, we describe the design and synthesis of an activity-based protein profiling probe and summarize our work toward developing a series of protocols for the labeling and visualization of 4′-phosphopantetheinylation of endogenous NRPSs in complex proteomes.

等待

The Assembly-Line Enzymology of Nonribosomal Peptide Biosynthesise chain before releasing the complete peptide. Adenylation, thiolation, condensation, and thioesterase domains play central roles in these reactions. This chapter focuses on the current understanding of these central domains in NRPS assembly-line enzymology.

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modifier

Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-Producing Non-ribosomal Pepobust analytical platform for A-domains and provides insights into their enzyme–substrate specificity. In this chapter, we describe the design and synthesis of these ABPP probes and provide a summary of our work on the development of a series of protocols for labeling, visualizing, and analyzing endogenous NRPSs in complex biological systems.