Titlebook: New Nucleic Acid Techniques; John M. Walker Book 1988 Springer Science+Business Media New York 1988

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考得

Computer Applications to Molecular Biology,ed, and the most recent issue of the . (release 10, 1987) contains almost 10 million base pairs in almost 9000 entries compared with the first release from 1982 containing half a million base pairs in 500 entries. The requirement for computers is obvious not only from the total amount of DNA sequenc

Chronological

Perigee

加强防卫

Manual Oligonucleotide Synthesis Using the Phosphoramidite Method,exocyclic amino groups on cytosine and the purines are protected as the alkali-labile benzoyl or .-butyryl amides. The 3′-OH group of the first nucleoside is attached to the solid support by a spacer chain, and the synthesis procedes by coupling the 5′-OH group of the growing chain to the 3′-phospho

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Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis,uction of novel linkers, site-directed mutagenesis, and many other techniques. The material obtained from a particular synthesis contains traces of premature termination products and other unwanted byproducts of the organic reactions. For most uses in genetic engineering, the material must be purifi

libertine

小卒

,Construction of Mammalian Genomic Libraries Using λ Replacement Vectors,ideal state can be approached by cutting the DNA randomly and cloning large pieces of this DNA into a suitable vector (.). The DNA can be cleaved either by mechanical shearing, in which case the DNA is fragmented in a truely random fashion, but with the introduction of problems associated with blunt

责难

Cosmid Library Construction,ely from bacteriophage λ, where nonessential intergenic regions were identified. It had been noted that only λ DNA 37–52 kb in length (or 78–102% of the wild-type length) were efficiently packaged and passaged in vivo. Thus vectors from which the intergenic region had been removed could be positivel

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