Titlebook: Neutrophil Methods and Protocols; Mark T. Quinn,Frank R. DeLeo,Gary M. Bokoch Book 2007 Humana Press 2007 Activation.Endoplasmatisches Ret

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Isolation of Human Neutrophils From Venous BloodVenous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies

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1064-3745 nd animal species.Presents an interesting and informative ov.Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disor

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Transduction of Proteins into Intact Neutrophilsn of a sequence derived from the HIV TAT protein to the protein to be introduced into the neutrophil. This requires both molecular biology to generate the initial construct as well as special procedures for protein isolation and renaturation. However, it also results in highly effective functional protein delivery into human neutrophils.

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Rac and Rap GTPase Activation Assaysy between GDP- and GTP-bound states and highly specific antibodies are not yet available for many small GTPases, analysis of certain members of the Rho and Ras GTPase family are now routinely performed. Here, we describe affinity-based pull-down assays for detection of Rac/Cdc42 and Rap activity in stimulated neutrophils.

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