Titlebook: Neuroprotection Methods and Protocols; Tiziana Borsello Book 2007 Humana Press 2007 Alzheimer.Endoplasmatisches Reticulum.Parkinson.Vivo.g

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书目名称Neuroprotection Methods and Protocols影响因子(影响力)




书目名称Neuroprotection Methods and Protocols影响因子(影响力)学科排名




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书目名称Neuroprotection Methods and Protocols网络公开度学科排名




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书目名称Neuroprotection Methods and Protocols被引频次学科排名




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书目名称Neuroprotection Methods and Protocols年度引用学科排名




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Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease Stateslatively inexpensive cost to assay a given brain metabolite makes this methodology useful in the interpretation of molecular and biochemical responses in terms of the condition of the tissue following a neurological insult.

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Brain on a Chip: A Method to Detect Novel Neuroprotective Candidate Targets, following data using GeneChip® technology (Affymetrix, Inc., Santa Clara, CA, USA), to identify, for example, potential brain neuroprotective targets. Moreover, step-by-step explanations of software operation will be provided. Finally, methods are presented to validate the gene expression changes revealed from the microarray analyses.

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Functional Cloning of Genes Regulating Apoptosis in Neuronal Cells,cribe also the screen of the library to identify genes regulating the processing of the β-amyloid (Aβ) precursor protein (APP), the protein associated with the pathogenesis of Alzheimer’s disease. The levels of the peptide Aβ, produced by the APP processing, are detected with ELISA based on the innovative Delfia method developed by PerkinElmer.

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A Modified Silver Technique (de Olmos Stain) for Assessment of Neuronal and Axonal Degeneration,d this method for several years to identify degenerating neurons in adult and neonatal rodent brains. A detailed protocol is provided, with attention to the most critical variables in tissue fixation and solution preparation. Examples are shown of axon injury in the rat brain after focal ischemia.

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Role of Nrf2-Dependent ARE-Driven Antioxidant Pathway in Neuroprotection,nt antioxidant gene therapy is performed to attenuate malonate-induced neuronal cell death. The details for generating Nrf2-overexpressing astrocytes and grafting them onto the lesion model are described in this chapter.

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