书目名称 | mRNA Decay | 副标题 | Methods and Protocol | 编辑 | Shireen R. Lamandé | 视频video | | 概述 | Includes cutting-edge methods and protocols for the study of mRNA decay.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .mRNA Decay: Methods and Protocols. serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.. | 出版日期 | Book 2018 | 关键词 | Decay pathways; mRNA-binding protein targets; Metabolic labeling; RNAseq; Bioinformatics; Nonsense-mediat | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-7540-2 | isbn_softcover | 978-1-4939-8515-9 | isbn_ebook | 978-1-4939-7540-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media LLC 2018 |
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