书目名称 | Mycobacteria Protocols | 编辑 | Tanya Parish,David M. Roberts | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory.. Mycobacteria Protocols, Third Edition .guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and up-to-date, .Mycobacteria Protocols, Third Edition. will be a resource both to those working in the field and to newcomers.. | 出版日期 | Book 2015Latest edition | 关键词 | Genetic manipulation; electroporation; gene expression; homologous recombination; homologous recombineer | 版次 | 3 | doi | https://doi.org/10.1007/978-1-4939-2450-9 | isbn_softcover | 978-1-4939-5582-4 | isbn_ebook | 978-1-4939-2450-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media New York 2015 |
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