Titlebook: Microtubules; Methods and Protocol Hiroshi Inaba Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

concession

书目名称Microtubules影响因子(影响力)




书目名称Microtubules影响因子(影响力)学科排名




书目名称Microtubules网络公开度




书目名称Microtubules网络公开度学科排名




书目名称Microtubules被引频次




书目名称Microtubules被引频次学科排名




书目名称Microtubules年度引用




书目名称Microtubules年度引用学科排名




书目名称Microtubules读者反馈




书目名称Microtubules读者反馈学科排名

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Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Usinnterference Contrast (FLIC) microscopy can illuminate the height of the microtubule above a surface, which, at sufficiently low surface densities of kinesin, also reveals the number, locations, and dynamics of the bound motors.

heterogeneous

Assembling Microtubule-Based Active Matterls and associated isotropic fluids. We describe the purification of each protein and the assembly process of a two-dimensional active nematic on a water–oil interface. Finally, we show examples of nematic formation and describe methods for quantifying their non-equilibrium dynamics.

可以任性

Book 2022ections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubul

CLASP

Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modificationis required. Additionally, fluorescently labeled tubulin has become a powerful approach for extensively studying the dynamics of these components. In this chapter, the process of purifying the heterodimeric αβ-tubulin from porcine brain will be described, as well as the process of labeling of the purified tubulin with fluorescence dye.

有偏见

Construction of Molecular Robots from Microtubules for Programmable Swarming of molecular robots through conjugation of DNA to MTs and demonstration of swarming of the MTs. The swarming is mediated by DNA-based interaction and photoirradiation which act as processors and sensors respectively for the robots. Furthermore, the required protocols to utilize the swarming of MTs for molecular computation is also described.

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