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Titlebook: Microscopy, Optical Spectroscopy, and Macroscopic Techniques; Christopher Jones,Barbara Mulloy,Adrian H. Thomas Book 1994 Springer Science

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High-Flux X-Ray and Neutron Solution Scatteringlthough X-rays are diffracted by electrons and neutrons are diffracted by nuclei, the physical principles are the same. Scattering views structures in random orientations to a nominal structural resolution of about 2–4 nm in a .* range (. = 4 π sin /g3/γ; 2/g3 = scattering angle; γ = wavelength) bet
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Determination of Macromolecular Homogeneity, Shape, and Interactions Using Sedimentation Velocity Anbiology, with applications ranging from simple purity checks and particle shape determinations from sedimentation velocity, isolation and purification of macromolecules using density gradient techniques right through to the evaluation of mol wt and mol-wt distributions, thermodynamic second virial c
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Determination of Absolute Molecular Weights Using Sedimentation Equilibrium Analytical Ultracentrifue molecular mass,” ... Despite this, it is not always easy to determine or, indeed, for a heterogeneous system, define. With a homogeneous system, the simplest procedure is to calculate it directly from the chemical formula-for example, for a protein whose amino acid composition or sequence is known
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The Measurement of Electronic Absorption Spectra in the Ultraviolet and Visibleters. The results of all measurements ought to be treated as “spectra” with the analyte absorption spectrum being the difference between the (analyte + solvent) spectrum and the solvent spectrum. Double beam spectrometers lend themselves to the traditional methodology involving matched cells, one co
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