书目名称 | MicroRNAs as Tools in Biopharmaceutical Production | 编辑 | Niall Barron | 视频video | | 概述 | Biology of microRNAs for use as tools.Implementation of these molecules to improve processes in the Biopharmceutical field.Methods to implement these molecules in the Bioprocess setting.Includes suppl | 图书封面 |  | 描述 | .Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.. | 出版日期 | Book 2012 | 关键词 | Bioprocessing; Chinese Hamster Ovary cells; MicroRNA; Recombinant protein production; genetic engineerin | 版次 | 1 | doi | https://doi.org/10.1007/978-94-007-5128-6 | isbn_softcover | 978-94-007-9526-6 | isbn_ebook | 978-94-007-5128-6 | copyright | Springer Science+Business Media Dordrecht 2012 |
The information of publication is updating
|
|