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Titlebook: MicroRNA and Cancer; Methods and Protocol Wei Wu Book 2018Latest edition Springer Science+Business Media LLC 2018 DNA damage.cancer-immune

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发表于 2025-3-21 17:05:32 | 显示全部楼层 |阅读模式
书目名称MicroRNA and Cancer
副标题Methods and Protocol
编辑Wei Wu
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: MicroRNA and Cancer; Methods and Protocol Wei Wu Book 2018Latest edition Springer Science+Business Media LLC 2018 DNA damage.cancer-immune
描述.This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .MicroRNA and Cancer: Methods and Protocols, Second Edition .aims to ensure successful results in the further study of this vital field.
出版日期Book 2018Latest edition
关键词DNA damage; cancer-immune system interaction; cancer cell resistance; APOBEC gene expression; gene expre
版次2
doihttps://doi.org/10.1007/978-1-4939-7435-1
isbn_softcover978-1-4939-8485-5
isbn_ebook978-1-4939-7435-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2018
The information of publication is updating

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发表于 2025-3-21 20:58:43 | 显示全部楼层
Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like Gene Expression, RNA Editing, and MNAs interact with APOBEC gene family, and post-transcriptionally regulate APOBEC gene expression in the APOBECA-B fusion allele and APOBEC-mediated RNA editing. It is reasonable to speculate targeting specific microRNAs may reduce host genome mutagenesis via inactivation of APOBEC deaminases.
发表于 2025-3-22 03:45:04 | 显示全部楼层
Angiogenesis Analysis by In Vitro Coculture Assays in Transwell Chambers in Ovarian Cancerion of precursor molecule in the tumorigenic SKOV3 ovarian cancer cell line, and analyzed the effects in cell migration, invasion, and tube formation of endothelial cells using matrigel-coated transwell chambers.
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MicroRNA, Noise, and Gene Expression Regulatione level, and stabilize mRNA and protein level to maintain normal cellular function. Furthermore, the changing of microRNA expression levels may increase the stochastic fluctuation leading to abnormal cellular function, even diseases.
发表于 2025-3-22 13:04:15 | 显示全部楼层
Identification of E6/E7-Dependent MicroRNAs in HPV-Positive Cancer CellsE7-dependent intracellular miRNAs by small RNA deep sequencing, upon silencing of endogenous . expression in HPV-positive cancer cells in vitro. In addition, we provide a protocol to identify E6/E7-dependent miRNA alterations in exosomes that are secreted by HPV-positive cancer cells in vitro.
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Application of Individual qPCR Performance Parameters for Quality Control of Circulating MicroRNA Dafication. Herein, we describe the protocol we have used to check the robustness of miRNA purification and measurement by the addition of spike-ins and by evaluating the quality of the qPCR data, respectively.
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