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Titlebook: MicroRNA Detection and Pathological Functions; Xueji Zhang,Haifeng Dong,Yaping Tian Book 2015 The Author(s) 2015 Cell imaging analysis.Ele

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发表于 2025-3-21 19:13:41 | 显示全部楼层 |阅读模式
书目名称MicroRNA Detection and Pathological Functions
编辑Xueji Zhang,Haifeng Dong,Yaping Tian
视频video
概述Provides a comprehensive summary of current techniques for miRNA detection.Describes both the technical and clinical aspects of miRNA.Addresses the promising clinical applications of miRNA, emphasizin
丛书名称SpringerBriefs in Molecular Science
图书封面Titlebook: MicroRNA Detection and Pathological Functions;  Xueji Zhang,Haifeng Dong,Yaping Tian Book 2015 The Author(s) 2015 Cell imaging analysis.Ele
描述.This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions..This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA researc
出版日期Book 2015
关键词Cell imaging analysis; Electrochemical genosensors; Functional nanoprobe; MiRNA biogenesis; MiRNA detect
版次1
doihttps://doi.org/10.1007/978-3-662-47293-4
isbn_softcover978-3-662-47292-7
isbn_ebook978-3-662-47293-4Series ISSN 2191-5407 Series E-ISSN 2191-5415
issn_series 2191-5407
copyrightThe Author(s) 2015
The information of publication is updating

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Conventional miRNA Detection Strategies, are basically and widely used technologies. As gold-standard methods, conventional miRNA detection strategies are continuously used for confirming results from newer detection techniques. For improving the sensitivity and reducing the throughput of these conventional strategies, numerous efforts we
发表于 2025-3-22 08:25:25 | 显示全部楼层
miRNA Electrochemical Detection,s. A typical electrochemical biosensor consists of an electroactive hybridization indicator and a solid electrode with an immobilized short single-stranded nucleotide probe [.]. Hybridization between the probe and the complementary sequence influences the electrochemical response signal, producing a
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Other Emerging miRNA Detection Strategies,t-generation sequencing, laser ablation (LA) inductively coupled plasma mass spectrometry (ICPMS), and so on. With a sensitive, rapid, and on-site analysis, SPR is an attractive alternative to conventional techniques in miRNA detection. Referring to small sample volume and rapid detection for POC di
发表于 2025-3-22 18:58:03 | 显示全部楼层
Intracellular and Organic miRNA In Situ Detection,urement of the expression levels of miRNAs in cells and tissues involves cells lysis and detection by qRT-PCR, northern blotting, or microarray hybridization. The direct analysis methods are a noninvasive manner for repetitively monitoring and obtaining real-time imaging of the intracellular miRNA b
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creasing by a fraction (1 − ., and successive maintenance times are independent and increasing by a fraction (. − 1), or alternatively, upon each repair the time to failure will be reduced to a fraction . of the immediately previous one and be independent of all previous ones and the repair time wil
发表于 2025-3-23 06:13:06 | 显示全部楼层
Xueji Zhang,Haifeng Dong,Yaping Tiancreasing by a fraction (1 − ., and successive maintenance times are independent and increasing by a fraction (. − 1), or alternatively, upon each repair the time to failure will be reduced to a fraction . of the immediately previous one and be independent of all previous ones and the repair time wil
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