Titlebook: Membrane Trafficking; Ales Vancura Book 2008 Humana Press 2008 DNA.Lipid.Mammalian cells.Membrane transport.Organelle.Translation.Yeast ce

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A Cell-Free System for Reconstitution of Transport Between Prevacuolar Compartments and Vacuoles in cuole in .. At least 20 of these genes function in transport between the prevacuolar endosome-like compartment (PVC) and the vacuole. To gain biochemical access to these functions, the authors developed a cell-free assay that measures transport-coupled proteolytic maturation of soluble zymogens .. A

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Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptidescell. A complex chain of reactions needs to be followed to achieve a successful transport of mitochondrial proteins from the cytosol through the double membrane system to their final destination. In order to elucidate the details of the translocation process, . import assays have been developed that

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Labeling and Analysis of Mitochondrial Translation Products in Budding and in Fission YeastsMitochondrial protein synthesis is unique on many respects, including the use of a genetic code with deviations from the universal code, the use of a restricted number of transfer RNAs, and because of the large number of nuclear encoded factors involved in assembly of the mitochondrial biosynthetic

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Exploring Protein-Protein Interactions Involving Newly Synthesized Mitochondrial DNA-Encoded Proteint, the structural subunits forming these multimeric enzymes are encoded in both genomes. In addition, the assistance of nuclear encoded factors, termed ., is necessary to allow for the expression of the mitochondrial DNA-encoded subunits and to facilitate their maturation, membrane insertion, and fu

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Using Quantitative Fluorescence Microscopy to Probe Organelle Assembly and Membrane Traffickingnce treated in a qualitative manner can now be treated quantitatively. We present here an overview of the principles involved in doing quantitative fluorescence microscopy. We illustrate these with examples drawn from our work with the Golgi apparatus and endosomes in cultured mammalian cells. The p