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Titlebook: Mass Spectrometry Data Analysis in Proteomics; Rune Matthiesen Book 2007 Humana Press 2007 Mascot.Proteomics.Translation.algorithms.bioinf

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Protein Identification by Tandem Mass Spectrometry and Sequence Database Searching,ly adopted. The identification of peptides from acquired MS/MS spectra is most often performed using the database search approach. We provide a detailed description of the peptide identification process and review the most commonly used database search programs. The appropriate choice of the search
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Quantitation With Virtual Expert Mass Spectrometrist,nt years. Particularly, labeling of peptides and proteins with stable isotopes such as .H, .N, and .C facilitated the unbiased comparison of protein amounts in distinct samples in a single mass spectrometric experiment. These methods can be applied to detect quantitative changes in protein amounts a
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Retention Time Prediction and Protein Identification,try. Separation methods, such as liquid chromatography and electrophoresis, are often used to fractionate complex protein or peptide mixtures and these separations also provide information on the different species, such as molecular weight and isoelectric point from electrophoresis and hydrophobicit
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Proteomic Data Exchange and Storage,cessibility. The further demands of multilaboratory initiatives has highlighted issues when collaborators cannot import data generated within the same project but generated by different hardware types and processed by laboratory-specific work flows and analyses packages. There is an increasing need
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