| 书目名称 | Long Non-Coding RNAs in Cancer | | 编辑 | Alfons Navarro | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... .Authoritative and practical, .Long Non-Coding RNAs in Cancer .aims to provide a collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field. . | | 出版日期 | Book 2021 | | 关键词 | lncRNAs; circRNAs; Real time PCR; ; viral vectors; Hydroximethylation | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-1581-2 | | isbn_softcover | 978-1-0716-1583-6 | | isbn_ebook | 978-1-0716-1581-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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发表于 2025-3-21 19:06:24
