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Titlebook: Live Cell Imaging; Methods and Protocol Sung-Bae Kim Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Passive o

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书目名称Live Cell Imaging
副标题Methods and Protocol
编辑Sung-Bae Kim
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Live Cell Imaging; Methods and Protocol Sung-Bae Kim Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Passive o
描述This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Live Cell Imaging: Methods and Protocols. aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science..
出版日期Book 2021
关键词Passive optical readouts; Bioluminescent probes; Functional substrates; Luciferases; Organic fluorescent
版次1
doihttps://doi.org/10.1007/978-1-0716-1258-3
isbn_softcover978-1-0716-1260-6
isbn_ebook978-1-0716-1258-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/587371.jpg
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Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteinsivision. To trace NE dynamics, the NE needs to be labeled with a fluorescent marker, and for this purpose, markers based on inner nuclear membrane (INM) proteins are normally used. However, NE labeling with INM proteins has some limitations. Here, we introduce a protocol for fluorescent labeling and
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Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Toolshways, the dynamics of which are modulated by intracellular trafficking. A significant contribution of β-arrestin to intracellular trafficking has been suggested, but the underlying mechanism is poorly understood. Here, we describe a protocol for manipulating β-arrestin-regulated membrane receptor t
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Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging in proliferative diseases, such as cancer. Developments in fluorescent biosensors have facilitated dynamic tracking of molecular processes, including the cell cycle. The genetically encoded set of fluorescent indicators, Fucci4, enables the visualization of transitions between each cell cycle phase
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