书目名称 | Lipoproteins and Cardiovascular Disease | 副标题 | Methods and Protocol | 编辑 | Lita A. Freeman | 视频video | | 概述 | Encompasses lipoprotein-centered methodologies designed for all experience levels.Features detailed protocols that are lab-ready.Includes expert tips and key implementation advice to ensure successful | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In .Lipoproteins and Cardiovascular Disease: Methods and Protocols., experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used. Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, r | 出版日期 | Book 2013 | 关键词 | Atherosclerosis; Gene knockout; Lipoprotein; RNA and gene expression; Sequencing; Transgenic models; Vascu | 版次 | 1 | doi | https://doi.org/10.1007/978-1-60327-369-5 | isbn_softcover | 978-1-4939-5690-6 | isbn_ebook | 978-1-60327-369-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2013 |
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