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Titlebook: Ion Mobility-Mass Spectrometry; Methods and Protocol Giuseppe Paglia,Giuseppe Astarita Book 2020 Springer Science+Business Media, LLC, part

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书目名称Ion Mobility-Mass Spectrometry
副标题Methods and Protocol
编辑Giuseppe Paglia,Giuseppe Astarita
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Ion Mobility-Mass Spectrometry; Methods and Protocol Giuseppe Paglia,Giuseppe Astarita Book 2020 Springer Science+Business Media, LLC, part
描述This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Ion Mobility-Mass Spectrometry: Methods and Protocols. serves as anideal resource for scientists delving into the technique‘s unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples..
出版日期Book 2020
关键词IM-MS; Informatics; Electrophoretic techniques; Complex biological sample analysis; Bioinformatics; Analy
版次1
doihttps://doi.org/10.1007/978-1-0716-0030-6
isbn_softcover978-1-0716-0032-0
isbn_ebook978-1-0716-0030-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
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Ion Mobility-Mass Spectrometry of Glycoconjugatesven valuable for improving structural assignments of individual glycans in complex biological mixtures. In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.
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Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled toy (IM) allows an additional level of separation and specificity to be obtained. Here, we describe the coupling of the technologies and the practical advantages of these combinations, highlighting specific examples.
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The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines source of the DMS-MS platform. Here we describe the application of FIA-DMS-MS/MS for the analysis of urinary acylcarnitine species. More than 20 acylcarnitine species can be detected and quantified during a single FIA-DMS-MS/MS acquisition.
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Liquid Extraction Surface Analysis (LESA) High-Field Asymmetric Waveform Ion Mobility Spectrometry ( separated according to their different mobilities in high and low electric fields. Here, we describe the protocols for LESA FAIMS mass spectrometry of intact proteins in thin tissue sections and bacterial colonies.
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