Titlebook: Integrative Structural Biology with Hybrid Methods; Haruki Nakamura,Gerard Kleywegt,John L. Markley Book 2018 Springer Nature Singapore Pt

谈判

View from Nuclear Magnetic Resonance Spectroscopyically, NMR spectroscopy was number two after X-ray crystallography in the rate of depositions to the Protein Data Bank (PDB). However, electron cryomicroscopy (CryoEM) recently surpassed NMR in this regard. NMR frequently is used in conjunction with X-ray or CryoEM in structure determinations. NMR

聪明

Complementary Use of Electron Cryomicroscopy and X-Ray Crystallography: Structural Studies of Actin e structure and dynamics of macromolecular complexes. Electron cryomicroscopy (cryoEM) and image analysis has become a powerful tool for structural studies because of recent technical developments in microscope optics, cryostage control, image detection and the methods of sample preparation. In part

ESO

Current Solution NMR Techniques for Structure-Function Studies of Proteins and RNA Moleculestroscopy, which enable hybrid methods. An advantage of NMR is that biomolecules can be studied at atomic resolution under near physiological conditions where they are dynamically active. We outline stable isotope labeling strategies, NMR data collection methodology, and procedures for data analysis

根除

The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biologya dodecapeptide that forms a β-turn in the binding pocket of NZ-1. This unique conformation allows for the PA peptide to be inserted into turn-forming loops within a folded protein domain and the system has been variously used in general applications including protein purification, Western blotting

Blemish

Small Angle Scattering and Structural Biology: Data Quality and Model Validationample preparation and instrumentation, (2) data reduction and analysis, and (3) three-dimensional structural modelling and validation. In this context, areas of ongoing research in regard to the interpretation of SAS data will be discussed with a particular focus on structural modelling using comput

下边深陷

Structural Investigation of Proteins and Protein Complexes by Chemical Cross-Linking/Mass Spectrometns and protein assemblies. Structural information is obtained by introducing covalent connections between amino acids that are in spatial proximity in proteins and protein complexes. The distance constraints imposed by the cross-linking reagent provide information on the three-dimensional arrangemen

人类

Creditee

A Hybrid Approach for Protein Structure Determination Combining Sparse NMR with Evolutionary Couplinis more challenging. An emerging hybrid strategy for modeling protein structures combines sparse NMR data that can be obtained for larger proteins with sequence co-variation data, called evolutionary couplings (ECs), obtained from multiple sequence alignments of protein families. This hybrid “EC-NMR

玛瑙

Maximizer