| 书目名称 | Inositol Phosphates and Lipids | | 副标题 | Methods and Protocol | | 编辑 | Christopher J. Barker | | 视频video | | | 概述 | Serves as an easily accessible reference volume for all aspects of measurement of inositol phosphates and lipids contributed by front-line researchers in the field.Combines in one volume methodologies | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In .Inositol Phosphates and Lipids: Methods and Protocols., expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls....Authoritative and cutting-edge, .Inositol Phosphates and Lipids: Methods and Protocols. compiles many of the techniques that underscore phos | | 出版日期 | Book 2010 | | 关键词 | Cellular regulation; Fluorescently labeled PH-domains; IP3 receptor; Inositol pyrophosphates; Intracellu | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-60327-175-2 | | isbn_softcover | 978-1-61779-689-0 | | isbn_ebook | 978-1-60327-175-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2010 |
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发表于 2025-3-21 18:46:54
