| 书目名称 | In Vitro Transcription and Translation Protocols | | 编辑 | Guido Grandi | | 视频video | | | 概述 | Completely updated edition with new chapters and topics.In-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems.Detailed protocols for protein pr | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps. . | | 出版日期 | Book 2007Latest edition | | 关键词 | ATP; Mutant; Mutation; PCR; Translation; genes; genetic information; molecular biology; proteins; transcripti | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-59745-388-2 | | isbn_softcover | 978-1-61737-641-2 | | isbn_ebook | 978-1-59745-388-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2007 |
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发表于 2025-3-21 17:21:11
