Titlebook: In Vitro Transcription and Translation Protocols; Martin J. Tymms Book 19951st edition Humana Press 1995

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Subtraction Hybridization cDNA Libraries,, tissues, cell lines) is different than in a second source. Single-stranded cDNAs from both sources are allowed to hybridize so that sequences common to the two sources will anneal. The annealed, double-stranded DNAs are “subtracted” from the hybridization solution, leaving a population of cDNA mol

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Quantitative Measurement of mRNA Using the RNase Protection Assay,nuclease T1 to digest single-stranded RNA, but not perfectly base-paired double-stranded RNA. In this respect RNA:RNA hybrids are more resistant to ribonuclease than RNA:DNA hybrids are to S1 nuclease, resulting in fewer artifacts. RNase protection has a number of advantages over Northern analysis i

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Vasodilation

Peak-Bone-Mass

DEI

An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNlan, mitomycin C, and mitoxantrone). Although the exact mechanism of action remains unresolved in most cases, the apparent role of DNA has prompted, over several decades, a wide range of studies of these drug-DNA interactions. The major objective of such studies has been to determine the structure o

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In Vitro Reconstitution of Progesterone-Dependent RNA Transcription in Nuclear Extracts of Human Brpecific gene networks in higher eukaryotes (.–.). The hormonal action is mediated through specific intracellular receptor proteins that are functionally inactive in the absence of the hormone ligand. The hormonal response is initiated by the high-affinity binding of a steroid ligand to its cognate r

OATH

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Microinjection of In Vitro Transcribed RNA and Antisense Oligonucleotides in Mouse Oocytes and Earlre very similar processes, oocytes could prove useful in elucidating the role of genes in cellular proliferation. Indeed, the identification of the involvement of c-. in meiosis is an excellent example of the manner in which oocytes have proven useful for establishing gene function .. c-. is the cel