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Titlebook: In Situ Hybridization: Medical Applications; G. R. Coulton,J. Belleroche Book 1992 Springer Science+Business Media Dordrecht 1992 Chromoso

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书目名称In Situ Hybridization: Medical Applications
编辑G. R. Coulton,J. Belleroche
视频videohttp://file.papertrans.cn/463/462993/462993.mp4
图书封面Titlebook: In Situ Hybridization: Medical Applications;  G. R. Coulton,J. Belleroche Book 1992 Springer Science+Business Media Dordrecht 1992 Chromoso
描述In situ hybridization has developed as a means of localizingspecific DNA and RNA sequences within tissues. The great strength ofthis approach is the ability to relate the distribution of specificnucleic acids with cell structures and the protein products of thetarget gene by means of immunohistochemistry. Complementary DNA, RNAor oligonucleotide probes, suitably labelled, are hybridized tospecific DNA or RNA targets within tissues. The spatial informationthus obtained has contributed greatly to our understanding of suchdiverse areas of research as gene mapping, viral infection,cytogenetics, protein synthesis, prenatal diagnosis and tissuegrafting. .This book is not intended as another recipe book, although it doesdescribe theoretical and practical aspects of the technology. Rather,the authors critically describe the contribution made by in situhybridization to specific areas of medical research. .
出版日期Book 1992
关键词Chromosom; DNA; genetics; hybridization; tissue
版次1
doihttps://doi.org/10.1007/978-94-011-2984-8
isbn_softcover978-94-010-5326-6
isbn_ebook978-94-011-2984-8
copyrightSpringer Science+Business Media Dordrecht 1992
The information of publication is updating

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Application of , hybridization to studies of messenger RNA in the nervous system: functional correle complexity of this tissue means that a technique with high resolving power is needed to identify changes in gene expression that occur in a small group of cells. The CNS is extremely heterogeneous, it contains a dozen classical transmitters and more than 40 neuropeptides all in discrete cell clust
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The use of , hybridization in studies of viral disease, of specific viral DNA or RNA sequences in a range of histological preparations, but by demonstrating the precise tissue, cellular and subcellular location of the virus it can correlate the presence of a virus with its pathological effects and provide insight into the mechanisms involved in virus-ho
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, hybridization for molecular cytogenetics, application for the microscopic detection of specific nucleic acid sequences (.; .; .). In contrast to the first microscopic work done with fluorescent antibody probes, the labels were radioisotopes, the detection of which was accomplished by micro-autoradiography. The disadvantages inherent to the
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Giorgio Terenghi,Julia M. Polakng corpses, are presented first. Then it is explained what the term reliable message transfer means. Next the principle of channel coding and decoding is described. The difference between the error correction by receiver and the automatic repeat request protocol is discussed, and the advantages and
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J. de Belleroche,L. Virgo,A. Rashid,Y. Collaço Moraesxplained. At first, the need for the message synchronisation is discussed, and also how this can be done and where it must be done. Then the notion of the transparent data transfer and the methods for its provision are presented; the difference between this provision in the respective cases of synch
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