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Titlebook: Impact: Design With All Senses; Proceedings of the D Christoph Gengnagel,Olivier Baverel,Stefan Weinzie Conference proceedings 2020 Springe

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Marie Paschke,Stefan Neuhäuser,Klaas De Rycke,Christoph Gengnagele protein of interest, are commonly measured using a triple quadrupole mass spectrometers operated in selected reaction monitoring (SRM) mode. More recently, the advances in targeted LC-MS/MS analysis based on parallel reaction monitoring (PRM) performed on a quadrupole-orbitrap instrument have allo
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Sayjel Vijay Patel,Nathan Kiatkulpibooneteins were qualitatively identified and 261 proteins were unambiguously quantified. These results showed that the clinical proteomic method using LCM coupled with ICAT and 2D-LC-MS/MS can carry out not only large-scale but also accurate qualitative and quantitative analysis.
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Christian Schmidts,Sven Pfeiffer,Omid Kokabi eventually the cleavage of the biotin tags. This chapter focuses on the ICAT labeling procedure itself, because sample preparation is the most critical step of an ICAT-based protein expression comparison experiment. Other related procedures such as the cation exchange high performance liquid chroma
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Anton Savov,Roger Winkler,Oliver Tessmanncreted vesicles from bone-forming osteoblast cells, enabling comprehensive profiles of their proteomes to be obtained by mass spectrometry. These methods can be easily adapted to study ECM and secreted vesicles in other cell types, primary cell cultures derived from animal models, or tissue specimen
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Seiichi Suzuki,Evy L. M. Slabbinck,Jan Knippersormatics enable to analyze the proteome of not more than 100–200 cells. Obviously, validation of result is essential. The present review describes and discusses the various methods developed to target cell populations of interest by laser microdissection, followed by analysis of their proteome.
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Geometric Degrees of Freedom and Non-conventional Spatial Structural Formsto control the design properties of the solutions. For instance, a synclastic, compression-only shell can turn into an anticlastic compression-and-tension combined shell with the same magnitude of internal forces and external loads reciprocal to the same force distribution/diagram (Fig. .). Using th
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Robotic Landscapes: Designing Formation Processes for Large Scale Autonomous Earth Movinge new applications and design methods for autonomous earth moving, a series of design studio has been implemented at the ETH Zurich as a collaboration between Professor Christophe Girot Chair of Landscape Architecture and Gramazio Kohler Research Chair of Architecture and Digital Fabrication. This a
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