Titlebook: Immunocytochemistry and Related Techniques; Adalberto Merighi,Laura Lossi Book 2015 Springer Science+Business Media New York 2015 adult ne

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The Evolution of Immunocytochemistry in the Dissection of Neural Complexity,. After a brief excursus along the history of the different techniques that led to substantial amelioration of the original indirect immunofluorescence protocol, we discuss here the main advantages and disadvantages of the individual techniques for the study of central and peripheral neurons, in par

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Immunohistochemical Analysis of Neuronal Networks in the Nervous System of ,ephalopodmolluscs. The protocols are designed for frozen and vibratomesections of the .brain, but are easily transferable to other cephalopod species and tissues, and are also specifically designed to detect small molecules such as monoamines. One of the two protocols has been adjusted to process pa

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Immunocytochemical Phenotype of Differentiating Neuronse specific labeling of neuroepithelial-deriving cells in the course of neurogenesis. On the basis of the time-dependent expression of several molecular signals named as early, intermediate, or late markers, neuroscientists can compose a coherent frame of sequential or overlapping neuronal phenotypes

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Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurothe adult brain. In most mammals, adult neurogenesis mainly occurs in the olfactory bulb and the hippocampal formation. Adult neurogenesis is thought to consist of several developmental stages that are characterized by morphological distinct cells, and these cells are known to express different mark

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Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogs: Benefits anU) are used extensively to study neuronal time of origin and pattern of migration. Here, we discuss the advantages and pitfalls of identifying dividing cells with these markers and emphasize that they simply indicate DNAsynthesis. Thus, in addition to dividing cells, they can label cells that are un

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