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Titlebook: Imaging, Manipulation and Optogenetics in Zebrafish; Itia Amandine Favre‐Bulle Book 2018 Springer Nature Switzerland AG 2018 In vivo Micro

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发表于 2025-3-21 19:12:28 | 显示全部楼层 |阅读模式
书目名称Imaging, Manipulation and Optogenetics in Zebrafish
编辑Itia Amandine Favre‐Bulle
视频video
概述Nominated as an outstanding Ph.D thesis by the University of Queensland, Sydney, Australia.Develops and combines novel optical methods for micromanipulation of neuronal system.Describes an intensively
丛书名称Springer Theses
图书封面Titlebook: Imaging, Manipulation and Optogenetics in Zebrafish;  Itia Amandine Favre‐Bulle Book 2018 Springer Nature Switzerland AG 2018 In vivo Micro
描述The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued..
出版日期Book 2018
关键词In vivo Micromanipulation; Optogenetics; Zebrafish Neurobiology; Optical Micromanipulation; Light Sheet
版次1
doihttps://doi.org/10.1007/978-3-319-96250-4
isbn_softcover978-3-030-07164-6
isbn_ebook978-3-319-96250-4Series ISSN 2190-5053 Series E-ISSN 2190-5061
issn_series 2190-5053
copyrightSpringer Nature Switzerland AG 2018
The information of publication is updating

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Optical Manipulation of Otoliths In-Vivo,d blood cells [.] and nanoparticles [.]. Those studies show that OT can trap and manipulate small objects in free flowing channels in relatively shallow tissue (50 .m) without any correction to the incoming beam.
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Conclusion, brain tissue, how we used those results for current and future studies, and finally how we used light and combined optical systems to overcome the invasive nature of pre-existing methods in the study of vestibular system in zebrafish.
发表于 2025-3-22 20:19:16 | 显示全部楼层
https://doi.org/10.1007/978-3-319-96250-4In vivo Micromanipulation; Optogenetics; Zebrafish Neurobiology; Optical Micromanipulation; Light Sheet
发表于 2025-3-22 22:04:53 | 显示全部楼层
Introduction,When aiming to understand the brain, its complex network and constitution, one has to develop tools to visualise and control the information flowing through it on a cellular level. Methods for studying the brain are flourishing through the development of many new experimental methods that are able to give answers to long-standing problems.
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Scattering in Zebrafish Brain for Optogenetics,In the previous chapter, I focussed on the theory and modelling of light scattering in brain tissue. In this chapter, I present the measurements of backscattered light in-vivo in the zebrafish brain and compare those with my Monte Carlo method and model.
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