Titlebook: Imaging and Tracking Stem Cells; Methods and Protocol Kursad Turksen Book 2020Latest edition Springer Science+Business Media, LLC, part of

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Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy,al alterations and eventually to the expression of distinct cellular biomarkers. Here, we provide a detailed step-by-step protocol to facilitate serial intravital multiphoton microscopy for long-term in vivo tracking of renal cells and subsequent ex vivo histology for immunohistological staining of

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Generation and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes and Endothelial Cells for Haracterize cell type-specific profiles of hiPSC-derived cardiomyocytes (hiPSC-CM) and endothelial cells (hiPSC-EC) at the cellular and subcellular levels. This can be utilized in a platform which can provide multiple endpoint profiles of candidate compounds.

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Covisualization of Global DNA Methylation/Hydroxymethylation and Protein Biomarkers for Ultrahigh-D5mC and 5hmC sites requires thorough denaturing of double-stranded DNA for antigen retrieval. The protocol we present overcomes this obstacle through optimization of the necessary cell processing to delineate cytosine variants and gDNA while preserving the three-dimensional (3-D) structure of the ce

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