Titlebook: Imaging and Tracking Stem Cells; Methods and Protocol Kursad Turksen Book 2020Latest edition Springer Science+Business Media, LLC, part of

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A Distinctive MRI-Based Absolute Bias Correction Protocol for the Potential Labelling and In Vivo Tplex biological pathways. Stem cell transplantation is an emerging self-deliverable therapeutic modality which could immensely improve the invigorating management of the problem. The synergistic interaction of the stem cells with the paracrine niche molecules at the site of injury is an end point th

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Efficient Labeling of Human Mesenchymal Stem Cells Using Iron Oxide Nanoparticles,se cells. Iron oxide labeling is one such uncomplicated noninvasive labeling method. These transformed nanocrystals can be used for varied applications including stem-cell tracking, magnetic resonance imaging, and theranostics. Here we elucidate the protocol for iron oxide nanoparticles synthesis (I

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Cell Cycle Analysis Using In Vivo Staining of DNA-Synthesizing Cells,ing DNA in the S-phase of the cell cycle. Availability of the anti-BrdU antibody clone MoBu-1 detecting only BrdU allowed to develop a method for the sequential DNA labelling by these two thymidine analogues for determining the cell cycle kinetic parameters..In the current step-by-step protocol, we

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Metabolic Labeling of Live Stem Cell for In Vitro Imaging and In Vivo Tracking,ain their full therapeutic potentials, the survival, viability, integration, homing, and differentiation of stem cells after transplant must be clearly understood. To meet these urgent needs, noninvasive stem cell imaging and tracking technologies have been developed. Metabolic labeling technique is

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Study of Intracellular Cargo Trafficking and Co-localization in the Phagosome and Autophagy-Lysosomich can contribute to some of the difficulties associated with these studies. By combining several approaches, we show how the trafficking and processing of photoreceptor outer segments in the phagosome and autophagy-lysosomal pathways of the retinal pigment epithelium (RPE) can easily be quantified

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Time-Lapse Video Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro,ffective therapeutic strategies for many diseases. However, a precise description of the biological events involved, such as proliferation, differentiation, cell fate decisions, migration, or viability, may be hampered by the classical use of experiments based on end-point analysis. By contrast, liv

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Multiphoton Microscopy for Noninvasive and Label-Free Imaging of Human Skin and Oral Mucosa Equivalls and/or dyes. It provides complimentary imaging modalities, which include two-photon excited fluorescence (2PEF) and second harmonic generation (SHG). 2PEF from endogenous chromophores such as nicotinamide adenine dinucleotides (NADH), flavins and keratin enable visualization of cellular and subce