书目名称 | Imaging Cell Signaling | 编辑 | Christoph Wuelfing,Robert F. Murphy | 视频video | http://file.papertrans.cn/462/461647/461647.mp4 | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume explores recent innovations across the entire pipeline of imaging signal transduction, cell preparation, cellular manipulation, image acquisition, and computational analysis. The chapters in this book cover topics such as rapid preparation of living Drosophila pupal macrophages for ex vivo imaging; controlling the potency of T cell activation using an optically tuneable chimeric antigen receptor; in situ imaging of proteins using DNA-PAINT super-resolution microscopy; reconstructing signaling networks using biosensor barcoding; and morphological, spatial, and dynamic models for cellular components. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and authoritative, .Imaging Cell Signaling. is a valuable resource that provides guidance to researchers looking to learn how to effectively tailor design projects to image, manipulate, and model signal transduction.. | 出版日期 | Book 2024 | 关键词 | iterative tissue staining; APEX-EM; correlative fluorescence; DNA origami; photoswitchable kinases | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-3834-7 | isbn_softcover | 978-1-0716-3836-1 | isbn_ebook | 978-1-0716-3834-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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