Titlebook: Hypoxia; Methods and Protocol L. Eric Huang Book 2018 Springer Science+Business Media, LLC 2018 ChIP.CRISPR.Cas9 Gene Editing.VHL binding.R

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Monitoring Functional Responses to Hypoxia in Single Carotid Body Cells,The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluorimetry and the patch-clamp technique.

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Immunohistochemistry of the Carotid Body,Immunohistochemistry (IHC) enables the detection and distribution of proteins in cells of tissues. IHC has become an indispensable approach for studying oxygen sensing by the carotid body (CB). This chapter provides a detailed description of IHC of CB tissue and isolated CB cells.

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L. Eric HuangIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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https://doi.org/10.1007/978-3-662-24697-9me. Herein, we outline protocols optimized to detect binding of Hypoxia-Inducible Factor (HIF)-1α or HIF-2α to putative hypoxia response elements (HREs) within HIF target genes expressed in breast tumor epithelial cells.

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