Titlebook: Human in Vitro Fertilization and Embryo Transfer; Don P. Wolf,Martin M. Quigley Book 1984 Plenum Press, New York 1984 Embryo.biology.ferti

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Enhanced Follicular Development with Clomiphene Citrate and Human Chorionic Gonadotropin, al. (1981) proved that clomiphene citrate given daily from days 5 through 9 of the menstrual cycle within the context of an IVF-ET program could result in viable pregnancies. We have elected to follow the latter approach in an attempt to achieve maturation of multiple pre-ovulatory follicles and to intervene in the biologic clock of ovulation.

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Laboratory Details in an , Fertilization and Embryo Transfer Program, modify them where appropriate. Additionally, references have been added throughout the chapter where modifications can be directly attributed to the contributions of others. IVF-ET is an exceedingly complex undertaking and the parameters which may contribute to success are virtually infinite.

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Fertility Potential Evaluation with the Zona-Free Hamster Egg Bioassay,eggs. In one, sperm are incubated with non-living human oocytes and the ability of sperm to penetrate the zona pellucida is measured (Overstreet and Hembree, 1976). The other, the zona-free hamster egg bioassay, utilizes the living naked egg of the hamster and sperm-egg fusion is the measured parameter (Yanagamachi et al., 1976).

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Patient Screening and Selection,hance of success with substantially less risk or expense to the patient. Therefore, pretreatment screening of the couple should insure that 1) there is no alternative therapy available, and 2) that they are treatable by IVF-ET.

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Sperm Capacitation,va. The conclusion reached by both investigators was that sperm must reside within the oviduct for some time prior to ovulation to acquire fertilizing ability. The acquisition of fertilizing ability, termed capacitation by Austin (1952), is now recognized as an essential feature of mammalian fertilization.

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The Clinical Coordinator in an , Fertilization and Embryo Transfer Program,. All this information must be organized and provided to the clinicians and laboratory scientists in a timely fashion, so they can plan the actual events of oocyte recovery, fertilization and embryo transfer.

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testifies to the successful clinical application of insights obtained from studies in reproductive biology over the last 20 years. In women, these studies have included: 1) characterization of the changes in blood hormone profiles throughout ovulatory menstrual cycles; 2) documentation of the hormon