Titlebook: Host-Fungal Interactions; Methods and Protocol Elaine Bignell Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021

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Analysis of Epithelial Cell Responses to Microbial Pathogens,ystander” cells with barrier function, the epithelial cell is now known to be a sentinel cell in the recognition and discrimination of commensal and pathogenic microorganisms and a key cell in initiating subsequent innate and adaptive immune responses. Here, we describe the main assays utilized in a

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Single-Cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluoreuman .irway .pithelial .ells (AECs) play a crucial role in host defense by promoting uptake and killing of inhaled microorganisms and concomitant cytokine production in order to recruit professional phagocytes to the site of infection. However, inhaled pathogens can also reside and replicate intrace

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,Fungal Bioreporters to Monitor Outcomes of ,: Host–Cell Interactions,nters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells t

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