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Titlebook: Hormone Assays in Biological Fluids; Michael J. Wheeler,J. S. Morley Hutchinson Book 2006 Humana Press 2006 Chromatography.Mass Spectromet

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Measurement of Glucocorticoids in Biological Fluids,hods are described for the collection, storage, and preparation of samples and for the immunoassay techniques for some common steroids in serum, urine, and saliva. Alternative methods (high-performance liquid chromatography, gas-liquid chromatography, Gas chromatography-mass spectrometry) that have
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Urinary Steroid Profiling,inborn errors of steroid metabolism, and useful in other disorders with altered steroid secretion. A general method is detailed. Steroids, mostly in the form of glucuronide and sulphate conjugates, are extracted using solid-phase cartridges, followed by enzymatic hydrolysis, reextraction of freed st
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