Titlebook: High-Throughput Screening Assays in Toxicology; Hao Zhu,Menghang Xia Book 2022Latest edition This is a U.S. government work and not under

大炮

Subdue

GFP-LC3 High-Content Assay for Screening Autophagy Modulators also be used to identify autophagy modulators by screening a large number of compounds. This chapter describes a cell-based high content green fluorescent protein (GFP)-LC3 assay using mouse embryonic fibroblasts (MEF) stably expressing GFP-LC3.

Anticonvulsants

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秘密会议

千篇一律

衰老

Cell-Based Imaging Assay for Detection of Phospholipidosiscreening (HTS) platform. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds that induce phospholipidosis.

correspondent

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Cell-Based Assays to Identify ERR and ERR/PGC Modulatorsgonists require examination of this nuclear receptor alone and together with PGC-1α. In this book chapter, we describe the step-by-step protocol of a multiplex luciferase assay designed to identify ERRα agonists, antagonists, and toxicity in one quantitative high-throughput screening assay using two different stable cell lines.

欲望

Cell-Based hERG Channel Inhibition Assay in High-Throughput Formattivity. This chapter describes a cell-based hERG channel inhibition assay that has been optimized and performed in a 1536-well plate format. The homogeneous and robust assay can be used to identify compounds that inhibit hERG channel activity.