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Titlebook: Helicobacter pylori Protocols; Christopher L. Clayton,Harry L. T. Mobley Book 1997 Springer Science+Business Media New York 1997 DNA.PCR.P

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PCR for the Detection of , in Gastric Juice Aspirates and Environmental Water Samples,y biopsy during gastroduodenoscopy. A positive culture or visualization of the organism by histologic examination of biopsy material can give a definitive diagnosis (.). To make a diagnosis without gastroduodenoscopy, indirect tests, such as ELISA or urea breath tests, must be relied on (.). The pol
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Defined Media for ,,ing its specific growth requirements, are still largely unknown, The organism is generally grown in complex media containing tissue extracts (e.g., of brain or heart) or proteolytic enzyme digests of meat or casein supplemented with blood or serum, which has made the metabolic pathways utilized by t
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Studies of Respiration in ,,ed oxygen tension (DOT) of cell suspensions or extracts. Continuous monitoring of DOT is achieved using a Clark-type oxygen electrode linked to a chart recorder. The volume of cell suspension required is of the order of 1 mL (0.5–1 mg cell protein mL) and the method is sufficiently sensitive to dete
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Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of ,,rst developed and proposed as a taxonomical tool for the identification and differentiation of bacteria by Grimont and Grimont, in 1986 (.). Since its introduction, ribotyping has found wide application in molecular epidemiology as a marker for a variety of bacterial genera, and has been successfull
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