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Titlebook: Glycoanalysis Protocols; Elizabeth F. Hounsell Book 1998Latest edition Humana Press 1998

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书目名称Glycoanalysis Protocols
编辑Elizabeth F. Hounsell
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: Glycoanalysis Protocols;  Elizabeth F. Hounsell Book 1998Latest edition Humana Press 1998
描述Now fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today‘s pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics...Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs ofboth biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.
出版日期Book 1998Latest edition
版次2
doihttps://doi.org/10.1385/0896033554
isbn_softcover978-1-61737-016-8
isbn_ebook978-1-59259-562-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1998
The information of publication is updating

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Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography,e cellular phenomena, assessment of the structure of sugar chains is indispensable. However, it is difficult to elucidate the structures of cell surface oligosaccharides because of two technical problems. First is the difficulty in fractionating various oligosaccharides heterogeneous in the number,
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Analysis of ,- and ,-Glycans by Pyridylamination,thod has been applied to structure analyses of glycans including glycosidase digestion (.), 2D-mapping by various kinds of high-performance liquid chromatography (HPLC) (.), partial acetolysis (.), Smith degradation (.), methylation analysis (.), nuclear magnetic resonance (.), mass spectrometry (.)
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Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins, spectroscopy, and rarely nuclear magnetic resonance spectroscopy. These methods required relatively large amounts of materials (micromoles), specialized training and experience, and in some cases, significant capital equipment outlays. Because of these restrictions, convenient carbohydrate analysts
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The Use of Cell and Organ Culture for the Study of Secreted Mucins,e of human colonic cell lines as a model of the adenoma-carcinoma sequence is of particular interest because it allows the changes in expression of mucins to be studied during the development and progression of disease (.,.). Recently, the importance of proliferation, differentiation, and apoptosis
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