Titlebook: Glyco-Engineering; Methods and Protocol Alexandra Castilho Book 2015 Springer Science+Business Media New York 2015 Carbohydrate composition

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Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Productncoded on a proofreading-deficient pol3 gene. This novel mutagenesis technique generates a pool of mutants that includes a no-mutant strain together with mutant strains carrying multiple mutations. By using a suitable screening system it is possible to isolate the desired mutant strain from this poo

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SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylationerences in numerous proteins involved in biosynthesis of the relevant glycan chains in these species. The need for efficient, robust and flexible methods for recombinant expression of proteins is met by the recently described MultiBac technology, an advanced and optimized baculovirus-based system fo

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Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-,-Acetylglucosaminidase and Ex β-.-acetylglucosaminidase (GlcNAcase), a hexosaminidase in the Golgi membrane which removes a terminal .-acetylglucosamine (GlcNAc), might contribute to simple N-glycosylation profile in several insect cells including . S2. Here, we describe GlcNAcase suppression strategy using RNA interference (RN

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N-Glyco-Engineering in Plants: Update on Strategies and Major Achievementse complex proteins from human origin, one issue that needs to be addressed is their glycan structure. In the past years we have been witnessing outstanding results in targeted manipulation of the plant .-glycosylation pathway allowing recombinant proteins to be produced with human-type oligosacchari

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Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typtowards a humanized form. Posttranslational modifications in plants are similar to those in humans, but several differences affect product quality and efficacy and can also cause immune responses in patients. In the moss . highly efficient gene targeting via homologous recombination enables glyco-en

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Heinz Rutishauser,Martin Gutknechtget organism. This methodology has been successfully applied towards enhancing the N-linked glycosylation efficiency of .. Here, we describe the generation of differentially sized libraries from the . W3110 genome followed by high-throughput semiquantitative glycan specific screening. DNA sequenced

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