Titlebook: Glutamate Receptors; Methods and Protocol Corinna Burger,Margaret Jo Velardo Book 2019 Springer Science+Business Media, LLC, part of Spring

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https://doi.org/10.1007/978-3-031-28722-0embranes and cytosol, synapses, and other intermediate cellular membranes from postmortem human brain tissue. These subcellular fractions can be used in a variety of downstream applications to assess the localization, relative abundance, and stoichiometry of glutamate receptor subunits along the forward trafficking pathway.

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懒洋洋

Pudendal-Nerve

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万神殿

Commodification and the Market,us Percoll-sucrose density gradients, collection of brain tissue, preparation of brain homogenates, isolation of synaptoneurosome bands from the discontinuous Percoll-sucrose gradients, and radiolabeling SN proteins. De novo protein synthesis can be reproducibly measured in SN prepared by this method.

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Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in thicity in acute hippocampal slices. While this chapter focuses on in vitro field electrophysiological investigations, many of the principles can be applied to single-cell recordings as well as in vivo electrophysiology and indeed many types of metaplasticity phenomena.

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Whole-Cell Patch-Clamp Electrophysiology to Study Ionotropic Glutamatergic Receptors and Their Roles) and N-methyl-.-aspartate (NMDA) receptors underlying behavior has been extensively studied. Here we will discuss commonly used protocols and techniques for performing whole-cell patch-clamp recordings and exploring AMPA and NMDA receptor-mediated glutamatergic responses and alterations in the context of substance abuse.

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Gene Expression Analysis by Multiplex Single-Cell RT-PCRerase chain reaction (PCR), how to design gene-specific probes, and how to validate them. This technique provides multiplexed expression data that cannot be easily obtained by other approaches such as immunological co-labeling.