Titlebook: Genotoxicity and DNA Repair; A Practical Approach L. María Sierra,Isabel Gaivão Book 2014 Springer Science+Business Media New York 2014 DNA

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Genotoxicity and DNA Repair978-1-4939-1068-7Series ISSN 1557-2153 Series E-ISSN 1940-6053

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https://doi.org/10.1007/978-3-663-04272-3put as compared to the standard format. The liquid system allows for processing several replicates at once with the possibility of using pipetting robots and has an easy colorimetric readout. The Ames II/Ames MPF also uses less S9 and produces less hazardous waste due to the low-volume multiwell format.

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Die Begriffsform im Mythischen Denken,echanism of micronucleus formation, presents practical guidelines for designing studies, and gives the step-by-step protocols of the in vivo micronucleus test in bone marrow and peripheral blood cells of rodents.

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https://doi.org/10.1007/978-3-642-92571-9terial. Here, we describe the standard alkaline version of the comet assay, both in vitro and in vivo, as well as the lesion-specific enzyme-modified comet assay (for detection of oxidized DNA lesions) to test nanoparticles. We also highlight critical points that need to be taken into consideration when assessing nanomaterial genotoxicity.

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https://doi.org/10.1007/978-3-662-42980-8ntal contaminants, and metals. The obtained results clearly confirm the usefulness of this combination (. and comet assay), and open its possibilities for a more widely use, selecting new cell targets and exposure scenarios..In this context, we present here detailed protocols to perform this assay using neuroblast and hemocyte cells.

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