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Titlebook: Genotoxicity Assessment; Methods and Protocol Alok Dhawan,Mahima Bajpayee Book 2019Latest edition Springer Science+Business Media, LLC, par

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书目名称Genotoxicity Assessment
副标题Methods and Protocol
编辑Alok Dhawan,Mahima Bajpayee
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Genotoxicity Assessment; Methods and Protocol Alok Dhawan,Mahima Bajpayee Book 2019Latest edition Springer Science+Business Media, LLC, par
描述This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as .in silico .approaches for genetic toxicology. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Genotoxicity Assessment: Methods and Protocols, Second Edition. serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences..
出版日期Book 2019Latest edition
关键词Genetic toxicology; Mutation assays; DNA damage; Chromosomal abnormalities; Toxicogenomics; Computational
版次2
doihttps://doi.org/10.1007/978-1-4939-9646-9
isbn_softcover978-1-4939-9648-3
isbn_ebook978-1-4939-9646-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biomes (Dic) and micronuclei (MN) in exposed individuals’ peripheral blood lymphocytes (PBL)..The present chapter does not claim to make an exhaustive and complete picture on the complex world of biodosimetry, to which a large number of specific guidelines for performing laboratory services by the Inte
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Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cellsrs to the use of ex vivo whole blood method, involving 72 h of culture with the block of cytokinesis starting at 44 h. BMNCyt protocol reports the established method for sample collection, processing, slide preparation and scoring.
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Micronucleus Analysis by Flow Cytometry of the in vitro MN test Organization for Economic Cooperation and Development (OECD) guideline 487 (OECD, Guideline for testing of chemicals: in vitro mammalian cell micronucleus test 487: in vitro mammalian cell micronucleus test (MNVIT). Organization for Economic Cooperation and Development, Pari
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The Comet Assay in Human Biomonitoringder to remove all cellular contents except the DNA attached to a nuclear scaffold. Subsequent electrophoresis results in accumulation of undamaged DNA sequences at the proximity of the nuclear scaffold, while damaged sequences migrate toward the anode. When visualized with fluorochromes, these migra
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Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkersing confocal immunofluorescence microscopy (LSCIM) of three-dimensionally preserved nuclei can be, quantitative and maintain the spatial information. Here we describe the experimental protocols to quantify individual key events of the DDR cascade in three-dimensionally preserved nuclei by LSCIM with
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Detection of DNA Double-Strand Breaks Using Pulsed-Field Gel Electrophoresislly relevant cellular processes as well as to investigate the genotoxic or clastogenic effects of toxicants. Pulsed-field gel electrophoresis (PFGE) is a widely used method for direct quantification of DNA DSBs. In this method, the cells exposed to DSB-inducing agents are embedded in the agarose blo
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