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Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2017 Springer Science+Business Media LLC 2017 enzymes.TALEN.ZFN.CRISPR.ze

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发表于 2025-3-21 16:53:20 | 显示全部楼层 |阅读模式
书目名称Genome Editing in Animals
副标题Methods and Protocol
编辑Izuho Hatada
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary material: .Includ
丛书名称Methods in Molecular Biology
图书封面Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2017 Springer Science+Business Media LLC 2017 enzymes.TALEN.ZFN.CRISPR.ze
描述.This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Genome Editing in Animals: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2017
关键词enzymes; TALEN; ZFN; CRISPR; zebra fish
版次1
doihttps://doi.org/10.1007/978-1-4939-7128-2
isbn_softcover978-1-4939-8393-3
isbn_ebook978-1-4939-7128-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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https://doi.org/10.1007/978-1-4615-9723-0 results revealed that at least five gRNA sequences, each of them having only one perfectly complementary site in the whole genome, could be designed for more than 95% of genes, regardless of the organism. Next, among those gRNAs, we selected gRNAs that did not have any other complementary site to t
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Stakeholders, Programmes and Strategies,mizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, var
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https://doi.org/10.1007/978-1-4615-9723-0the CRISPR/Cas system, the RNA-guided endonuclease Cas protein introduces a DNA double-stranded break at the genome position recognized by a guide RNA (gRNA) based on complementary base-pairing of about 20-nucleotides in length. The 8- or 12-mer gRNA sequence in the proximal region is especially imp
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