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Titlebook: Genetically Encoded Functional Indicators; Jean-René Martin Book 2012 Springer Science+Business Media, LLC 2012 Brain visualization.Calciu

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发表于 2025-3-21 17:42:56 | 显示全部楼层 |阅读模式
书目名称Genetically Encoded Functional Indicators
编辑Jean-René Martin
视频video
概述Presents a comprehensive overview of the biophysical and biochemical properties of the titular probes.Features easy and accessible descriptions of the main imaging tools and methods now available.Incl
丛书名称Neuromethods
图书封面Titlebook: Genetically Encoded Functional Indicators;  Jean-René Martin Book 2012 Springer Science+Business Media, LLC 2012 Brain visualization.Calciu
描述.In the last decade, several different optical imaging techniques, either based on various voltage or calcium dyes, or more recently on modified fluorescent or bioluminescent proteins (genetically encoded) that are sensitive to calcium, have been developed to study neuronal activity, and especially groups of neurons, with the goal of mapping and deciphering the neural code underlying major neurophysiological functions.  .Genetically Encoded Functional Indicators. brings together expert contributors to present the development of recent genetic techniques that allow for generating genetically encoded activity sensors in order to investigate neuronal activity. Each chapter describes a specific sensor and its utilization to study neuronal activity in a particular way.  Written in the .Neuromethods. series style, chapters contain the kind of key description and implementation advice that guarantees successful results.. .Helpful and easy to use, .Genetically Encoded Functional Indicators. aims to inspire students and researchers and to serve as a useful guide to those who wish to start using these different brain imaging techniques and require a bit of guidance in how best to choose a te
出版日期Book 2012
关键词Brain visualization; Calcium imaging; Neural circuits; Neuroimaging; Neuronal activity; Neurophysiology
版次1
doihttps://doi.org/10.1007/978-1-62703-014-4
isbn_softcover978-1-4939-5938-9
isbn_ebook978-1-62703-014-4Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media, LLC 2012
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Calcium Imaging of Neural Activity in the Olfactory System of Drosophila,ation is subsequently encoded and processed by an overlapping, combinatorial activity of neurons forming complex neural circuits in the brain. In order to functionally dissect this neural circuitry, optical recording techniques allow visualizing spatial as well as temporal aspects of odor representa
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Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin,ar memory traces are defined as any change in the activity of a neuron that is induced by learning, which subsequently alters the processing and response of the nervous system to the sensory information that is learned. Synapto-pHluorin (spH), a protein fusion of a pH-sensitive mutant of green fluor
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In Vivo Optical Recording of Brain Interneuron Activities from a Drosophila Male on a Treadmill,ous organisms, enabling the exploration of neural substrates for complex behaviors. As a showcase for such renovated neuroethology with GECIs, we describe a novel method to record optically interneuron activities from the brain of a tethered male fruit fly . when contact chemosensory stimulation is
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Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies two-photon imaging of neural activity in an essentially intact fly. We present strategies to minimize motion of the brain, both in preparation technique and in apparatus design. We discuss key variables for reducing the problems of photobleaching and phototoxicity in order to collect high quality
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Engineering and Application of Genetically Encoded Calcium Indicators,n chimeric proteins consisting of a recognition element (e.g., a ligand-binding protein) and a reporter element (one or more fluorescent proteins). The analyte-induced conformational change in the recognition element leads to an observable change in fluorescence in the reporter element. Expression o
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Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps,unction. The development of genetically encoded sensors for cAMP has made it possible to measure cAMP dynamics within living cells with high temporal and spatial resolution. In this chapter, we review live-imaging methods for the measurement of relative cAMP levels within single neurons of the expla
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