Titlebook: Exocytosis and Endocytosis; Methods and Protocol Florence Niedergang,Nicolas Vitale,Stéphane Gasman Book 2021 Springer Science+Business Med

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Funktionssysteme der Gesellschaftteins that have reached the Golgi apparatus via the retrograde route from those that have not. The choice of antibodies or natural ligands allows to adjust these methods to virtually any chosen biological system.

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Designing for Fun: User-Testing Case Studiesscopy. Further, we discuss the level of complexity, costs, and the reasons why a researcher should choose each technique..This chapter is designed to provide the user with sufficient background to measure acrosomal exocytosis in human sperm.

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https://doi.org/10.1007/978-3-030-57835-0ombination with selective protein immobilization (SPI). SPI is based on the coating of coverslips with anti-green fluorescent protein (GFP) antibodies, which capture the GFP-tagged RUSH cargos once exposed to the cell surface after its release by the addition of biotin.

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https://doi.org/10.1007/978-3-662-68720-8hat are applicable to study the internalization of flaviviruses; dextrans; transporters, such as, glutamate transporter vGlut1; and peptidergic signaling molecules, including atrial natriuretic peptide into astrocytes, the most heterogeneous neuroglial cells, which play a key homeostatic role in the central nervous system.

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