Titlebook: Exocytosis and Endocytosis; Andrei I. Ivanov Book 2014Latest edition Springer Science+Business Media New York 2014 biochemical assays.cult

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Reinhard Pöschel,Lev A. Kalužninfor GPCRs. These proteins have the obvious advantage that their fluorescence can be switched once the GPCR of interest has reached a specific subcellular compartment. Here, we summarize the recent progress for live cell imaging of GPCRs using these PCFPs for trafficking, biosynthesis, and protein/protein interaction studies.

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Ziel und Zweck des Forschungsvorhabens, from a broad range of commonly used fluorescent proteins. Here we describe how to establish new tFTs and consider potential pitfalls. We detail a protocol for quantitative fluorescence microscopy imaging and analysis of intracellular protein dynamics with tFTs in the budding yeast ..

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Bewegungsanalytische Verfahren,SH) system enabling simultaneous and synchronous release of secretory cargos from the endoplasmic reticulum in a population of cells. Here, we describe how to subclone the gene coding for a cargo of interest into a RUSH plasmid and to monitor its synchronized transport along the secretory pathway in fixed samples and in living cells.

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https://doi.org/10.1007/978-3-642-72361-2UT4. We found that sialylated glycoforms of GFP-tagged GLUT4 appear to be internalized more slowly than non-sialylated GLUT4 upon insulin removal. This novel glycan imaging tool allows probing functional roles of specific glycan modifications in endocytosis of various proteins.

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Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptidesning peptides to facilitate downstream mass spectrometry (MS) identification of lysine ubiquitination sites. This approach can be utilized to identify ubiquitination sites from proteins in a complex mixture.

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Use of Kaede and Kikume Green–Red Fusions for Live Cell Imaging of G Protein-Coupled Receptorsfor GPCRs. These proteins have the obvious advantage that their fluorescence can be switched once the GPCR of interest has reached a specific subcellular compartment. Here, we summarize the recent progress for live cell imaging of GPCRs using these PCFPs for trafficking, biosynthesis, and protein/protein interaction studies.

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