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Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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发表于 2025-3-21 17:41:38 | 显示全部楼层 |阅读模式
书目名称Epiblast Stem Cells
副标题Methods and Protocol
编辑Pierre Osteil
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license
描述This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful .Method in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Epiblast Stem Cells: Methods and Protocols. serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast..
出版日期Book 2022
关键词EpiSC; Human developmental steps; Pluripotent stem cells; Epiblast-like cells; Primordial germ cells; Bio
版次1
doihttps://doi.org/10.1007/978-1-0716-2281-0
isbn_softcover978-1-0716-2283-4
isbn_ebook978-1-0716-2281-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313140.jpg
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978-1-0716-2283-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Epiblast Stem Cells978-1-0716-2281-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Air Pollution Effects on Biodiversityotency is a continuum of different states between the two extremes of naïve embryonic stem cells (ESCs) and primed Epiblast Stem Cells (EpiSCs), which resemble the pre/peri- and post- implantation embryo, respectively. The transition from naïve to primed pluripotency can be induced by growing naïve
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https://doi.org/10.1007/978-1-4757-4465-1 development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigen
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Perry J. Samson,Jennie L. Moodyue to innate differences in cellular characteristics, the efficiency of lipid-based transfection is variable across cell types. Pluripotent cells which exist in a “primed” state such as human embryonic stem cells (hESCs) and mouse epiblast stem cells (mEpiSCs) are notorious for being refractory to l
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